The Manual of Clinical Microbiology is considered the most authoritative reference in the field. It has been indispensable to clinical microbiologists, laboratory technologists, and infectious disease specialists for 40 years, enabling them to accurately detect clinically significant bacteria, viruses, fungi, and parasites. This 10th edition represents the collaborative efforts of 22 editors and more than 260 authors from around the world, all experienced researchers and practitioners in medical and diagnostic microbiology. Together, they have brought the manual fully up to date, producing a remarkable work of two volumes, nine sections, and 149 chapters that is filled with the latest research findings, infectious agents, methods, practices, and safety guidelines.

 

The 10th edition of the Manual of Clinical Microbiology continues to set the standard for state-of-the-science laboratory practice. It begins with a section dedicated to core diagnostic strategies and topics. Next, it helps readers understand the underlying mechanisms of both common and emerging infectious agents and diseases. With its careful step-by-step guidance, the Manual enables readers to correctly perform the appropriate diagnostic, therapeutic, and susceptibility tests and then interpret the results. Rounding out its coverage, the Manual addresses the taxonomy, biology, epidemiology, transmission, and clinical significance of almost all microorganisms that cause disease in humans.

 

With its all-encompassing, up-to-date coverage, the Manual of Clinical Microbiology is the ideal starting point to begin addressing almost any question in the field. For most questions, researchers typically find everything they need within the Manual. Detailed references at the end of each chapter serve as a gateway to the primary literature, enabling researchers to fully investigate more complex questions.

 

Fully updated and revised, this edition of the Manual of Clinical Microbiology encapsulates the knowledge, practices, and procedures of the field, enabling readers to accurately detect pathogens and diagnose infectious disease.

James Versalovic, Texas Children's Hospital

 

Karen C. Carroll, The Johns Hopkins Hospital

Guido Funke, Gärtner and Colleagues Laboratories

James H. Jorgensen, University of Texas Health Science Center at San Antonio

Marie Louise Landry, Yale University School of Medicine

David W. Warnock, Centers for Disease Control and Prevention

 

Ronald M. Atlas, University of Louisville

Kathryn A. Bernard, Public Health Agency of Canada

Mary E. Brandt, Centers for Disease Control and Prevention

Angela M. Caliendo, Emory University Hospital

J. Stephen Dumler, The Johns Hopkins Hospital

Lynne S. Garcia, LSG & Associates

Christine C. Ginocchio, North Shore-LIJ Health System Laboratories

Elizabeth M. Johnson, The HPA Centre for Infections (UK)

Jean B. Patel, Centers for Disease Control and Prevention

Cathy A. Petti, Stanford University School of Medicine

Gary W. Procop, Cleveland Clinic

Sandra S. Richter, Cleveland Clinic

Yi-Wei Tang, Vanderbilt University Medical Center

Alexandra Valsamakis, The Johns Hopkins Hospital

Peter Vandamme, Universiteit Gent (Belgium)

Melvin P. Weinstein, Robert Wood Johnson Medical School

 

 

1.   Introduction to the Tenth Edition of the Manual of Clinical Microbiology / James Versalovic

2.   Microscopy / Danny L. Wiedbrauk

3.   Systems for Detection and Identification of Bacteria and Yeasts / Cathy A. Petti, Melvin P. Weinstein, and Karen C. Carroll

4.   Molecular Microbiology / Frederick S. Nolte and Angela M. Caliendo

5.   Immunoassays for the Diagnosis of Infectious Diseases / A. Betts Carpenter

6.   Infection Control Epidemiology and Clinical Microbiology / Daniel J. Diekema and Michael A. Pfaller

7.   Investigation of Enteric Disease Outbreaks / Timothy F. Jones and William E. Keene

8.   Molecular Epidemiology / Peter Gerner-Smidt, Eija Hyytiä-Trees, and Paul A. Rota

9.   Procedures for the Storage of Microorganisms / Cathy A. Petti and Karen C. Carroll

10. Prevention and Control of Laboratory-Acquired Infections / Michael A. Noble

11. Decontamination, Disinfection, and Sterilization / Andreas F. Widmer and Reno Frei

12. Biothreat Agents / Susan E. Sharp and Mike Loeffelholz

13. The Human Microbiome / Sarah K. Highlander, James Versalovic, and Joseph F. Petrosino

14. Microbial Genomics and Pathogen Discovery / Anne M. Gaynor and David Wang

 

 

15. Taxonomy and Classification of Bacteria / Peter A. R. Vandamme

16. Specimen Collection, Transport, and Processing: Bacteriology / Ellen Jo Baron and Richard B. Thomson, Jr.

17. Reagents, Stains, and Media: Bacteriology / Ronald M. Atlas and James W. Snyder

 

18. General Approaches to Identification of Aerobic Gram-Positive Cocci / Kathryn L. Ruoff

19. Staphylococcus, Micrococcus, and Other Catalase-Positive Cocci / Karsten Becker and Christof von Eiff

20. Streptococcus / Barbara Spellerberg and Claudia Brandt

21. Enterococcus / Lúcia Martins Teixeira, Maria da Glória Siqueira Carvalho, Patricia Lynn Shewmaker, and Richard R. Facklam

22. Aerococcus, Abiotrophia, and Other Aerobic Catalase-Negative, Gram-Positive Cocci / Kathryn L. Ruoff

 

23. General Approaches to the Identification of Aerobic Gram-Positive Rods / Kathryn A. Bernard

24.  Bacillus and Other Aerobic Endospore-Forming Bacteria / Niall A. Logan, Alex R. Hoffmaster, Sean V. Shadomy, and Kendra E. Stauffer

25. Listeria and Erysipelothrix / Nele Wellinghausen

26.Coryneform Gram-Positive Rods / Guido Funke and Kathryn A. Bernard

27. Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and Other Aerobic Actinomycetes / Patricia S. Conville and Frank G. Witebsky

28. Mycobacterium: General Characteristics, Laboratory Detection, and Staining Procedures / Gaby E. Pfyffer and Frantiska Palicova

29. Mycobacterium: Laboratory Characteristics of Slowly Growing Mycobacteria / Elvira Richter, Barbara A. Brown-Elliott, and Richard J. Wallace, Jr.

30. Mycobacterium: Clinical and Laboratory Characteristics of Rapidly Growing Mycobacteria / Barbara A. Brown-Elliott and Richard J. Wallace, Jr.

 

31. Approaches to the Identification of Aerobic Gram-Negative Bacteria / Georges Wauters and Mario Vaneechoutte

32. Neisseria / Johannes Elias, Matthias Frosch, and Ulrich Vogel

33. Actinobacillus, Capnocytophaga, Eikenella, Kingella, Pasteurella, and Other Fastidious or Rarely Encountered Gram-Negative Rods / Reinhard Zbinden and Alexander von Graevenitz

34. Haemophilus / Nathan A. Ledeboer and Gary V. Doern

35. Escherichia, Shigella, and Salmonella / James P. Nataro, Cheryl A. Bopp, Patricia I. Fields, James B. Kaper, and Nancy A. Strockbine

36. Yersinia / Martin E. Schriefer and Jeannine M. Petersen

37. Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and Other Enterobacteriaceae / Sharon L. Abbott

38. Aeromonas / Amy J. Horneman and Afsar Ali

39. Vibrio and Related Organisms / Sharon L. Abbott, J. Michael Janda, and J. J. Farmer III

40. Pseudomonas / Deborah A. Henry and David P. Speert

41. Burkholderia, Stenotrophomonas, Ralstonia, Cupriavidus, Pandoraea, Brevundimonas, Comamonas, Delftia, and Acidovorax / John J. LiPuma, Bart J. Currie, Sharon J. Peacock, and Peter A. R. Vandamme

42. Acinetobacter, Chryseobacterium, Moraxella, and Other Nonfermentative Gram-Negative Bacteria / Mario Vaneechoutte, Lenie Dijkshoorn, Alexandr Nemec, Peter Kämpfer, and Georges Wauters

43. Bordetella and Related Genera / Carl-Heinz Wirsing von König, Marion Riffelmann, and Tom Coenye

44. Francisella and Brucella / Jeannine M. Petersen, Martin E. Schriefer, and George F. Araj

45. Legionella / Paul H. Edelstein

46. Bartonella / Ricardo G. Maggi, Volkhard A. J. Kempf, Bruno B. Chomel, and Edward B. Breitschwerdt

 

47. Approaches to Identification of Anaerobic Bacteria / Ellen Jo Baron

48. Peptostreptococcus, Finegoldia, Anaerococcus, Peptoniphilus, Veillonella, and Other Anaerobic Cocci / Yuli Song and Sydney M. Finegold

49. Propionibacterium, Lactobacillus, Actinomyces, and Other Non-Spore-Forming Anaerobic Gram-Positive Rods / William G. Wade and Eija Könönen

50. Clostridium / Dennis L. Stevens, Amy E. Bryant, Anja Berger, and Christoph von Eichel-Streiber

51. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and Other Anaerobic Gram-Negative Rods / Eija Könönen, William G. Wade, and Diane M. Citron

 

52. Algorithms for Identification of Curved and Spiral-Shaped Gram-Negative Rods / Irving Nachamkin

53. Campylobacter and Arcobacter / Collette Fitzgerald and Irving Nachamkin

54. Helicobacter / Andrew J. Lawson

55. Leptospira / Paul N. Levett

56. Borrelia / Martin E. Schriefer

57. Treponema and Brachyspira, Human Host-Associated Spirochetes / Justin D. Radolf, Allan Pillay, and David L. Cox

 

58. General Approaches to Identification of Mycoplasma, Ureaplasma, and Obligate Intracellular Bacteria / J. Stephen Dumler

59. Mycoplasma and Ureaplasma / Ken B. Waites and David Taylor-Robinson

60. Chlamydiaceae / Charlotte Gaydos and Andreas Essig

61. Rickettsia and Orientia / David H. Walker and Donald H. Bouyer

62. Ehrlichia, Anaplasma, and Related Intracellular Bacteria / Megan E. Reller and J. Stephen Dumler

63. Coxiella / Stephen R. Graves and Robert F. Massung

64. Tropheryma / Thomas Marth

 

 

65. Antibacterial Agents / Joseph D. C. Yao and Robert C. Moellering, Jr.

66. Mechanisms of Resistance to Antibacterial Agents / Louis B. Rice and Robert A. Bonomo

67. Susceptibility Test Methods: General Considerations / John D. Turnidge, Mary Jane Ferraro, and James H. Jorgensen

68. Susceptibility Test Methods: Dilution and Disk Diffusion Methods / Jean B. Patel, Fred C. Tenover, John D. Turnidge, and James H. Jorgensen

69. Susceptibility Testing Instrumentation and Computerized Expert Systems for Data Analysis and Interpretation / Sandra S. Richter and Mary Jane Ferraro

70. Special Phenotypic Methods for Detecting Antibacterial Resistance / Jana M. Swenson, Jean B. Patel, and James H. Jorgensen

71. Susceptibility Test Methods: Fastidious Bacteria / Janet A. Hindler and James H. Jorgensen

72. Susceptibility Test Methods: Anaerobic Bacteria / Diane M. Citron and David W. Hecht

73. Susceptibility Test Methods: Mycobacteria, Nocardia, and Other Actinomycetes / Gail L. Woods, Shou-Yean Grace Lin, and Edward P. Desmond

74. Detection and Characterization of Antimicrobial Resistance Genes in Pathogenic Bacteria / J. Kamile Rasheed and Fred C. Tenover

 

 

 

 

75. Taxonomy and Classification of Viruses / Elliot J. Lefkowitz

76. Specimen Collection, Transport, and Processing: Virology / Michael S. Forman and Alexandra Valsamakis

77. Reagents, Stains, Media, and Cell Cultures: Virology / Christine C. Ginocchio and Patricia C. Harris

78. General Approaches for Detection and Identification of Viruses / Marie Louise Landry, Angela M. Caliendo, Christine C. Ginocchio, Yi-Wei Tang, and Alexandra Valsamakis

 

79. Human Immunodeficiency Viruses / Brigitte P. Griffith, Sheldon Campbell, and Angela M. Caliendo

80. Human T-Cell Lymphotropic Virus Types 1 and 2 / S. Michele Owen, Antoine Gessain, Charlene S. Dezzutti, Elliot P. Cowan, and Renu B. Lal

81. Influenza Viruses / Robert L. Atmar and Stephen E. Lindstrom

82. Parainfluenza and Mumps Viruses / Diane S. Leland

83. Respiratory Syncytial Virus and Human Metapneumovirus / Yi-Wei Tang and James E. Crowe, Jr.

84. Measles and Rubella Viruses / William J. Bellini and Joseph P. Icenogle

85. Enteroviruses and Parechoviruses / Kathleen A. Stellrecht, Daryl M. Lamson, and José R. Romero

86. Rhinoviruses / Marie Louise Landry

87. Coronaviruses / Kanti Pabbaraju and Julie D. Fox

88. Hepatitis A and E Viruses / David A. Anderson and Natalie A. Counihan

89. Hepatitis C Virus / Michael S. Forman and Alexandra Valsamakis

90. Gastroenteritis Viruses / Xiaoli Pang and Xi Jiang

91. Rabies Virus / Lillian A. Orciari and Charles E. Rupprecht

92. Hendra and Nipah Viruses / Pierre E. Rollin, Paul A. Rota, Sherif Zaki, and Thomas G. Ksiazek

93. Arboviruses / Robert S. Lanciotti and Theodore F. Tsai

94. Hantaviruses / Charles F. Fulhorst and Michael D. Bowen

95. Arenaviruses and Filoviruses / Pierre E. Rollin, Stuart T. Nichol, Sherif Zaki, and Thomas G. Ksiazek

 

96. Herpes Simplex Viruses and Herpes B Virus / Keith R. Jerome and Rhoda Ashley Morrow

97. Varicella-Zoster Virus / Elisabeth Puchhammer-Stöckl and Stephan W. Aberle

98. Human Cytomegalovirus / Richard L. Hodinka

99. Epstein-Barr Virus / Barbara Gärtner

100.  Human Herpesviruses 6, 7, and 8 / Philip E. Pellett and Graham Tipples

101.  Adenoviruses / Christine Robinson and Marcela Echavarria

102.  Human Papillomaviruses / Patti E. Gravitt and Christine C. Ginocchio

103.  Human Polyomaviruses / Richard S. Buller

104.  Human Parvoviruses / Jeanne A. Jordan

105.  Poxviruses / Inger K. Damon

106.  Hepatitis B and D Viruses / Rebecca T. Horvat and Gary E. Tegtmeier

 

107.  Transmissible Spongiform Encephalopathies / Adriano Aguzzi and Markus Glatzel

 

 

108.  Antiviral Agents / Nell S. Lurain and Kenneth D. Thompson

109.  Mechanisms of Resistance to Antiviral Agents / Robert W. Shafer, Isabel Najera, and Sunwen Chou

110.  Susceptibility Test Methods: Viruses / Max Q. Arens and Ella M. Swierkosz

 

 

111.  Taxonomy and Classification of Fungi / Mary E. Brandt and David W. Warnock

112.  Specimen Collection, Transport, and Processing: Mycology / Karin L. McGowan

113.  Reagents, Stains, and Media: Mycology / James W. Snyder, Ronald M. Atlas, and Mark T. LaRocco

114.  General Approaches for Direct Detection of Fungi / Yvonne R. Shea

 

115.  Candida, Cryptococcus, and Other Yeasts of Medical Importance / Susan A. Howell and Kevin C. Hazen

116.  Pneumocystis / Melanie T. Cushion

117.  Aspergillus and Penicillium / S. Arunmozhi Balajee and Mary E. Brandt

118.  Fusarium and Other Opportunistic Hyaline Fungi / Deanna A. Sutton and Mary E. Brandt

119.  Agents of Systemic and Subcutaneous Mucormycosis and Entomophthoromycosis / Dea Garcia-Hermoso, Eric Dannaoui, Olivier Lortholary, and Françoise Dromer

120.  Histoplasma, Blastomyces, Coccidioides, and Other Dimorphic Fungi Causing Systemic Mycoses / Mary E. Brandt, Beatriz L. Gomez, and David W. Warnock

121.  Trichophyton, Microsporum, Epidermophyton, and Agents of Superficial Mycoses / Richard C. Summerbell

122.  Bipolaris, Exophiala, Scedosporium, Sporothrix, and Other Melanized Fungi / Josep Guarro and G. Sybren de Hoog

123.  Fungi Causing Eumycotic Mycetoma / Abdalla O. A. Ahmed and G. Sybren de Hoog

124.  Mycotoxins / Nancy C. Isham, William J. Halsall, and Mahmoud A. Ghannoum

125.  Lacazia, Pythium, and Rhinosporidium / Leonel Mendoza and Raquel Vilela

 

 

126.  Antifungal Agents / David W. Warnock

127.  Mechanisms of Resistance to Antifungal Agents / Theodore C. White and Samantha G. Hoot

128.  Susceptibility Test Methods: Yeasts and Filamentous Fungi / Elizabeth M. Johnson, Ana V. Espinel-Ingroff, and Michael A. Pfaller

 

 

129.  Taxonomy and Classification of Human Parasitic Protozoa and Helminths / Francis E. G. Cox

130.  Specimen Collection, Transport, and Processing: Parasitology / Robyn Y. Shimizu, Felix Grimm, Lynne S. Garcia, and Peter Deplazes

131.  Reagents, Stains, and Media: Parasitology / Andrea J. Linscott and Susan E. Sharp

132.  General Approaches for Detection and Identification of Parasites / Lynne S. Garcia, Robyn Y. Shimizu, and Graeme P. Paltridge

 

133.  Plasmodium and Babesia / William O. Rogers

134.  Leishmania and Trypanosoma / David A. Bruckner and Jaime A. Labarca

135.  Toxoplasma / James B. McAuley, Jeffery L. Jones, and Kamaljit Singh

136.  Pathogenic and Opportunistic Free-Living Amebae /  Govinda S. Visvesvara

137.  Intestinal and Urogenital Amebae, Flagellates, and Ciliates / Amy L. Leber and Susan Novak-Weekley

138.  Isospora, Cyclospora, and Sarcocystis / David S. Lindsay, Steve J. Upton, and Louis M. Weiss

139.  Cryptosporidium / Lihua Xiao and Vitaliano Cama

140.  Microsporidia / Rainer Weber, Peter Deplazes, and Alexander Mathis

141.  Nematodes / Harsha Sheorey, Beverley-Ann Biggs, and Peter Traynor

142.  Filarial Nematodes / Doran L. Fink and Thomas B. Nutman

143.  Cestodes / Hector H. Garcia, Juan A. Jimenez, and Hermes Escalante

144.  Trematodes / Malcolm K. Jones, Jennifer Keiser, and Donald P. McManus

145.  Less Common Helminths / Gary W. Procop and Ronald C. Neafie

146.  Arthropods of Medical Importance / Sam R. Telford III

 

 

147.  Antiparasitic Agents / Karin Leder and Peter F. Weller

148.  Mechanisms of Resistance to Antiparasitic Agents /  W. Evan Secor and Jacques Le Bras

149.  Susceptibility Test Methods: Parasites / Jacques Le Bras and W. Evan Secor